**部分 顯微技術(shù)和微生物形態(tài)學(xué)part one microscopic techniques and microbial morphology 實驗一 明視野光學(xué)顯微鏡 experiment 1 bright--field light microscope 實驗二 暗視野光學(xué)顯微鏡 experiment 2 dark--field light microscope 實驗三 單染色法 experiment 3 simple stain 實驗四 革蘭染色法 experiment 4 gram's stain 實驗五 芽孢染色法 experiment 5 spore stain 實驗六 莢膜染色法 experiment 6 capsule stain 實驗七 鞭毛染色法 experiment 7 flagella stain 實驗八 螺旋體的染色和形態(tài)觀察 experiment 8 spirochetes morphology 實驗九 懸滴法觀察細(xì)菌的動力 experiment 9 observation of bacterial motion with hanging drop 實驗十 酵母菌的形態(tài)觀察 experiment 10 yeast morphology 實驗十一 微生物大小的測定 experiment 11 determination of the size of microorganisms 實驗十二 放線菌、霉菌的菌絲和孢子形態(tài)觀察 experiment 12 morphology of mycelia and spore for actinomycetes and molds 實驗十三 細(xì)菌、放線菌、酵母菌和霉菌的菌落特征觀察 experiment 13 colony characteristics of bacteria,actinomycetes, yeasts and molds第二部分 基本實驗操作與培養(yǎng)技術(shù)part two basic laboratory and culture techniques 實驗十四 培養(yǎng)基的配制與滅菌 experiment 14 culture media preparation and sterilization 實驗十五接種技術(shù)與微生物的分離純化 experiment 15 culture transfer techniques and isolation of pure cultures 實驗十六 常用的消毒滅菌方法 experiment 16 commonly used sterilization and disinfection methods 實驗十七 菌種保藏 experiment 17 preservation of pure cuhures 實驗十八 微生物計數(shù)法 experiment 18 counting of microorganisms 實驗十九 細(xì)菌的生長曲線測定 experiment 19 bacteria growth curve第三部分 細(xì)菌的生理生化反應(yīng) part three biochemical activities of bacteria 實驗二十 淀粉水解和明膠液化試驗 exeriment 20 hydrolysis of starch and protein 實驗二十一 糖發(fā)酵試驗 exeriment 21 utilization of carbohydrates 實驗二十二 imvic試驗 exeriment 22 imvic series 實驗二十三 硫化氫產(chǎn)生試驗和觸酶試驗 exeriment 23 hydrogen sulfide production and catalase test第四部分 病毒學(xué)實驗part four experiments for virology 實驗二十四 流感病毒的培養(yǎng)與檢測 experiment 24 culture and detection of influenza virus 實驗二十五 噬菌體分離和效價測定 experiment 25 isolation and titer detection of bacteriophage 實驗二十六 噬菌體一步生長曲線的測定 experiment 26 one——step growth curve of bacteriophage第五部分 微生物遺傳學(xué)實驗part five experiments for microbial genetics 實驗二十七 微生物的遺傳與變異現(xiàn)象 experiment 27 heredity and variation of microorganism 實驗二十八 微生物抗藥性變異 experiment 28 drug resistant variation 實驗二十九 組氨酸營養(yǎng)缺陷型菌株的誘變和篩選 expreiment 29 mutagenesis and isolation of his auxotroph 實驗三十 鼠傷寒沙門菌/微粒系統(tǒng)檢測化學(xué)誘變劑 experiment 30 ames test:a bacterial test system for detecting mutagens andcarcinogens 實驗三十一抗藥質(zhì)粒接合傳遞實驗 experiment 31 bacterial conjugation:the transfer of antibiotic--resistant plasmids 實驗三十二 抗藥質(zhì)粒dna的分離檢測和轉(zhuǎn)化 experiment 32 isolation and characterization of p1asmid dna and transformation 實驗三十三 細(xì)菌轉(zhuǎn)導(dǎo) experiment 33 bacterial transduction 實驗三十四 枯草桿菌原生質(zhì)體的制備和再生 experiment 34 formation and regeneration ofbacillus subtilis protoplast 實驗三十五 利用16s rrna基因序列進(jìn)行細(xì)菌分類鑒定 experiment 35 bacterial identification with 16srrna gene第六部分 免疫學(xué)實驗part six experiments of immunology 實驗三十六 抗原與免疫血清的制備 experiment 36 preparations of antigen and antiserum 實驗三十七 吞噬細(xì)胞功能的測定 experiment 37 assay of the function of phagocytes 實驗三十八 體液因子的免疫功能測定 experiment 38 assay of immune functions of humoral factors 實驗三十九 體液免疫功能檢測(1) experiment 39 immune functions by antibody(1) 實驗四十 體液免疫功能檢測(2) experiment40 immune functions of antibodies(2) 實驗四十一 細(xì)胞免疫功能檢測(1)——體外實驗 experiment 41 immune function by cellular immunity(1)——in vitro 實驗四十二 細(xì)胞免疫功能檢測(2)——細(xì)胞因子的檢測 experiment 42 immune function by cellular immunity(2) ——detection of cytokines levels 實驗四十三 細(xì)胞免疫功能檢測(3)——體內(nèi)實驗 experiment 43 immune function by cellular immunity(3)——in vivo第七部分 藥學(xué)微生物學(xué)實驗part seven general mierobial experiments in pharmaeeutics 實驗四十四 藥物的體外抗菌試驗 experiment 44 determination of antimicrobial activityin vitro 實驗四十五 抗生素效價測定 experiment 45 assay of antibiotic potency 實驗四十六 藥物的無菌檢查法 experiment 46 sterility test of drugs 實驗四十七 藥物的微生物限度檢查法 experiment 47 microbial quality and microbial limit tests 0f pharmaceutical preparations第八部分 綜合性實驗part eight integrated experiments 實驗四十八土壤中抗生素產(chǎn)生菌的分離、鑒定和抗菌活性測定 experiment 48 isolation and identification of antibiotic-producing actinomycetes from soil,and the determination of its antimicrobial activity 實驗四十九 重組人干擾素-α工程菌的構(gòu)建、培養(yǎng)和誘導(dǎo)表達(dá) experiment 49 construction expression and cuhivation of recombinant e.coli expressing inf-α 實驗五十 產(chǎn)淀粉酶枯草桿菌的選育 experiment 50 screening ofbacillus subtilis with high produetivitv of amylase 實驗五十一 產(chǎn)β-內(nèi)酰胺酶抗藥細(xì)菌的分離和鑒定 experiment 51 isolation and identification of β-lactamase producing staphylococcus aureus 實驗五十二污水中大腸埃希菌噬菌體的分離、培養(yǎng)和效價測定 experiment 52 isolation,cultivation and titration ofescherichia coli phage from sewage第九部分 設(shè)計性實驗part nine designed experiments 實驗五十三 藥品的微生物學(xué)質(zhì)量標(biāo)準(zhǔn)的建立 experiment 53 microbial quality control of pharmaceuticals 實驗五十四 營養(yǎng)缺陷型菌株的篩選和鑒定 experiment 54 isolation and identification of auxotroph 實驗五十五 葡萄中酵母菌的分離、培養(yǎng)和鑒定 experiment 55 isolation,culture and identification 0f yeast in grapes 實驗五十六 菌種的復(fù)壯 experiment 56 strain reiuvenation 實驗五十七 基因工程菌種子庫的構(gòu)建 experiment 57 establishmerit of seed bank of genetic engineered bacteria 實驗五十八 環(huán)境中微生物的檢測 experiment 58 detection of environmental microorganisms 實驗五十九 利用biolog自動分析系統(tǒng)分離鑒定人體正常菌群 experiment 59 isolation and identification of normal flora in human body using biolog automatic analysis system 實驗六十 藥物對流感病毒的抑制作用 experiment 60 in vitro inhibition of influenza virus by antiviral drugs 實驗六十一 實驗藥物對機(jī)體的免疫調(diào)節(jié)作用 experiment 61 regulatory effects of pharmaceuticals on immunity 實驗六十二 抗原抗體反應(yīng)在傷寒疫苗制備中的應(yīng)用 experiment 62 application of antigen—antibody reaction in preparation of typhoid vaccine附錄 ⅰ.常用無菌玻璃儀器的準(zhǔn)備和廢品處理 ⅱ.教學(xué)用菌種 ⅲ.常用培養(yǎng)基 ⅳ.染色液的配制 ⅴ.試劑及緩沖液配制appendix ⅰ.sterilization of glass apparatus and treatment of waste ⅱ.microorganisms ⅲ.culture media ⅳ.staining solutions ⅴ.solutions and buffers專業(yè)名詞表words and vocabulary參考文獻(xiàn)(references)