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微章宏愿: 李季倫科研團隊論文選集(上下冊) 版權信息
- ISBN:9787030767691
- 條形碼:9787030767691 ; 978-7-03-076769-1
- 裝幀:圓脊精裝
- 冊數:暫無
- 重量:暫無
- 所屬分類:>
微章宏愿: 李季倫科研團隊論文選集(上下冊) 內容簡介
本書為中國科學院院士李季倫科研論文著作選集。李季倫為中國農業大學生物學院教授。自1950年至今,一直工作在教學、科研**線。本書分別從四個主要的方面(生物固氮的研究、微生物次生代謝產物研發及生物合成調控研究、真菌毒素及生物能源的研究和趨磁螺菌及其納米磁小體的研究),通過這四個方面的大約200余篇科學論文,記錄了李季倫院士的科研思想和科研歷程,以及對微生物學科所作出的學術貢獻。由于論文數量較多,本書只選擇性地收錄了部分研究論文,充分體現了李季倫教授敏銳的科研洞察力和腳踏實地的科研特質,展示了他理論聯系實際,為社會和大眾服務的科研理念,善于發現和解決問題的工作作風;團結協作,勇于攻關的無私奉獻精神。
微章宏愿: 李季倫科研團隊論文選集(上下冊) 目錄
目錄
序
前言
上
第1部分 生物固氮研究
我國生物固氮研究的現狀和對策 4
花生根瘤菌自生固氮的研究 8
耐鹽高效大豆根瘤菌株的構建 15
苜蓿根瘤菌 (Rhizobium meliloti) 的耐鹽性研究 21
紅豆草根瘤菌的耐鹽和共生性狀改造 28
Tn5-Mob系統誘導根瘤菌屬之間耐鹽和共生性狀的轉移 33
北京郊區固氮螺菌的研究 38
酶聯免疫吸附技術(ELISA)對大豆根瘤菌的鑒定 46
肺炎克氏桿菌的nifA基因產物在巴西固氮螺菌中的功效 52
巴西固氮螺菌(Azospirillum brasilense)Yu62的質粒及nif HDK基因的定位 61
肺炎克氏桿菌nifA基因在巴西固氮螺菌固氮基因表達的銨調節中的作用 67
幾種固氮菌nifA基因片段的同源性分析 71
巴西固氮螺菌ntrBC基因的克隆與核苷酸序列分析 76
肺炎克氏桿菌NifA對巴西固氮螺菌nifH啟動子的轉錄激活作用 83
巴西固氮螺菌Yu62 draTG基因啟動子區域的核苷酸序列及其功能分析 89
巴西固氮螺菌Yu62 draTG基因及其下游區域的定位誘變分析 97
巴西固氮螺菌Yu62 nifA基因克隆、測序及功能分析 103
巴西固氮螺菌鞭毛調節基因flbD鑒定 109
巴西固氮螺菌Yu62 glnB基因的克隆及其功能分析 115
玉米聯合固氮工程菌的增產和節約氮肥的效果 122
Interaction Between PII and NifA in Azospirillum brasilense Sp7 126
Functional Analysis of the GAF Domain of NifA in Azospirillum brasilense: Effects of Tyr → Phe Mutations on NifA and Its Interaction with GlnB 133
PAS Domain of the Deduced Org35 Protein Mediates the Interaction with NifA 144
Isolation of a Flagellar Operon in Azospirillum brasilense and Functional Analysis of FlbD 149
Interaction Between GlnB and the N-terminal Domain of NifA in Azospirillum brasilense 161
A Minimal Nitrogen Fixation Gene Cluster from Paenibacillus sp. WLY78 Enables Expression of Active Nitrogenase in Escherichia coli 171
Comparative Genomic Analysis of N2-Fixing and Non-N2-Fixing Paenibacillus spp.: Organization, Evolution and Expression of the Nitrogen Fixation Genes 188
Positive and Negative Regulation of Transferred nif Genes Mediated by Indigenous GlnR in Gram-positive Paenibacillus polymyxa 212
Combined Assembly and Targeted Integration of Multigene for Nitrogenase Biosynthetic Pathway in Saccharomyces cerevisiae 234
Influence of pN2 and pD2 on HD Formation by Various Nitrogenases 248
固氮酶催化的放H2反應 265
肺炎克氏桿菌固氮酶雙突變株的構建及其對底物還原的特性 275
N2 和H+在固氮酶活性中心金屬原子簇中還原位點的分析 285
Mutagenesis at α-423Ile of MoFe Protein Reduces the Catalytic Activity of Nitrogenase in Klebsiella oxytoca 294
α-Lys424 Participates in Insertion of FeMo-co to MoFe Protein and Maintains Nitrogenase Activity in Klebsiella oxytoca M5al 305
第2部分微生物次生代謝產物研發及生物合成調控研究
北京農業大學的赤霉素研究工作(簡報) 326
赤霉素A4、A7的發酵研究 330
赤霉素A9檢定方法的建立和發酵的初步研究 337
莫能菌素應用技術 343
透明顫菌血紅蛋白基因在阿維鏈霉菌中的表達 345
阿維菌素高產菌株的選育及阿維菌素B1的鑒定 352
Avermectins發酵培養基的研究 358
阿維鏈霉菌中aveD基因缺失對阿維菌素合成的影響 366
Deletion Analysis of Oligomycin PKS Genes (olmA) in Streptomyces avermitilis 371
Construction of Ivermectin Producer by Domain Swaps of Avermectin Polyketide Synthase in Streptomyces avermitilis 380
An adpA Homologue in Streptomyces avermitilis is Involved in Regulation of Morphogenesis and Melanogenesis 392
Engineering of Avermectin Biosynthetic Genes to Improve Production of Ivermectin in Streptomyces avermitilis 403
Enhancement of Avermectin and Ivermectin Production by Overexpression of the Maltose ATP-binding Cassette Transporter in Streptomyces avermitilis 410
A New Strain of Streptomyces avermitilis Produces High Yield of Oligomycin A with Potent Anti-tumor Activity on Human Cancer Cell Lines in vitro 423
Chromosomal Instability in Streptomyces avermitilis: Major Deletion in the Central Region and Stable Circularized Chromosome 433
The Pathway-specific Regulator AveR from Streptomyces avermitilis Positively Regulates Avermectin Production while it Negatively Affects Oligomycin Biosynthesis 450
Increasing Avermectin Production in Streptomyces avermitilis by Manipulating the Expression of a Novel TetR-family Regulator and Its Target Gene Product 466
AvaR2, a Pseudo γ-Butyrolactone Receptor Homologue from Streptomyces avermitilis, is a Pleiotropic Repressor of Avermectin and Avenolide Biosynthesis and Cell Growth 490
SAV742, a Novel AraC-Family Regulator from Streptomyces avermitilis, Controls Avermectin Biosynthesis, Cell Growth and Development 513
AvaR1, a Butenolide-type Autoregulator Receptor in Streptomyces avermitilis, Directly Represses Avenolide and Avermectin Biosynthesis and Multiple Physiological Responses 532
Redox-sensing Regulator Rex Regulates Aerobic Metabolism, Morphological Differentiation, and Avermectin Production in Streptomyces avermitilis 552
The PhoP Transcription Factor Negatively Regulates Avermectin Biosynthesis in Streptomyces avermitilis 568
IdeR, a DtxR-family Iron-response Regulator, Controls Iron Homeostasis, Morphological Differentiation, Secondary Metabolism, and Oxidative Stress Response in Streptomyces avermitilis 583
AveI, an AtrA Homolog of Streptomyces avermitilis, Controls Avermectin and Oligomycin Production, Melanogenesis, and Morphological Differentiation 604
SufR, a [4Fe-4S] Cluster-containing Transcription Factor, Represses the sufRBDCSU Operon in Streptomyces avermitilis Iron-sulfur Cluster Assembly 621
The ROK-family Regulator Rok7B7 Directly Controls Carbon Catabolite Repression, Antibiotic Biosynthesis, and Morphological Development in Streptomyces avermitilis 642
AccR, a TetR Family Tra
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