Content Chapter 1 Introduction to Basic Safety in Bio-Laboratory 4 1.1 Introduction to biosafety levels and general precautions in bioscience laboratory 4 1.2 Safety guidelines on water usage in bioscience laboratory 6 1.3 Safety guidelines on electricity usage in bioscience laboratory 8 1.4 Safety guidelines on gas usage in bioscience laboratory 10 1.5 Safety guidelines on sound usage in bioscience laboratory 12 1.6 Safety guidelines on light usage in bioscience laboratory 13 Chapter 2 Standard Operations and Safety Precautions of General Apparatus in Bio-Laboratory 16 2.1 Standard operations and safety precautions of autoclave usage.......................................................................................................16 2.2 Standard operations and safety precautions of oven usage 20 2.3 Standard operations and safety precautions of ultra-low temperature freezer 22 2.4 Standard operations and safety precautions of liquid nitrogen container 23 2.5 Standard operations and safety precautions of centrifuge .......................................................................................................24 2.6 Standard operations and safety precautions of biosafety cabinet 28 2.7 Standard operations and safety precautions of clean bench 30 2.8 Standard operations and safety precautions of fume hood ........................................................................................................32 2.9 Standard operations and safety precautions of UV analyzer 33 2.10 Standard operations and safety precautions of laser 35 2.11 Standard operations and safety precautions of laser power meter 37 2.12 Standard operation and safety precaution of biological signal acquisition and analysis system 39 Chapter 3 General Safety Guidelines for Common Biochemical Reagents Usages 42 3.1 Toxic reagents 42 3.2 Chemical unstable reagents 49 3.3 Animal anesthetics 53 Chapter 4 Storage and Handling Guidelines of Experimental Biomaterials 55 4.1 Storage and handling guideline of biomaterials in the life science laboratory 55 4.2 Blood samples of human and animal 61 4.3 Microorganism 63 4.4 Biomacromolecule 64 Chapter 5 Bio-science Safety, Emergency Care and First Aid 66 5.1 Bio-laboratory potential fire accidents 66 5.2 Explosive Accidents 69 5.3 Poisoning and burn accidents 71 5.4 First aid medical box in bio-laboratory 75 References 76